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    <ns1:expectedDelivery xml:lang="en">D (more than 6 months)</ns1:expectedDelivery>
    <ns10:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/15496443"/>
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    <ns0:brs_depositor xml:lang="en">Itaru IMAYOSHI</ns0:brs_depositor>
    <ns0:brs_depositor xml:lang="en">Ryuichiro KAGEYAMA</ns0:brs_depositor>
    <skos:altLabel xml:lang="ja">B6-Hes3 KO &amp; Hes5 KO (LacZ knock-in)  ; B6-Hes3/Hes5 KO</skos:altLabel>
    <skos:altLabel xml:lang="en">B6-Hes3 KO &amp; Hes5 KO (LacZ knock-in)  ; B6-Hes3/Hes5 KO</skos:altLabel>
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    <ns5:termsAndConditions xml:lang="en">In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2004 131(22):5539-50.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.  The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama  (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).</ns5:termsAndConditions>
    <ns0:brs_depositor xml:lang="ja">今吉　格</ns0:brs_depositor>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/15496443"/>
    <dcterms:identifier>RBRC06043</dcterms:identifier>
    <ns0:brs_depositor xml:lang="ja">影山龍一郎</ns0:brs_depositor>
    <ns1:donorDNA>Neo (E. coli), EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES),beta-galactosidase (E. coli), mouse phosphoglycerate kinase promoter (PGK promoter),  mouse Hes3 genomic DNA, Nuclear-localization signal from SV40 largeT antigen, Puromycin resistance gene (Streptomyces alboniger), mouse Hes5 genomic DNA</ns1:donorDNA>
    <ns0:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainType_23"/>
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    <ns1:strainDevelopment xml:lang="ja">京都大学ウィルス研究所・影山龍一郎（2000）。TT2 ES細胞由来を用いて作出。C57BL/6と交配。</ns1:strainDevelopment>
    <rdfs:label xml:lang="en">B6.Cg-Hes3&lt;tm1Kag&gt; Hes5&lt;tm1Kag&gt;</rdfs:label>
    <rdfs:label>B6.Cg-Hes3&lt;tm1Kag&gt; Hes5&lt;tm1Kag&gt;</rdfs:label>
    <ns1:ESCellLine>TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]</ns1:ESCellLine>
    <dc:description xml:lang="en">Hes3 and Hes5 double knockout mice.  The Hes3 gene was disrupted by a lac-neo cassette.  The Hes5 gene was disrupted by IRES-nls-lacZ expression cassette.</dc:description>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
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    <dc:description xml:lang="ja">Hes3およびHes5遺伝子のダブルノックアウトマウス。Hes3遺伝子はlacZ-neoカセットにより破壊、Hes5遺伝子がIRES-核移行LacZとPuromycine耐性遺伝子により破壊されている。ヘテロで維持する場合は、両遺伝子が同じ姉妹染色体に乗っている事を確認する必要がある。</dc:description>
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    >true</ns1:isRecombinant>
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    <ns1:strainDevelopment xml:lang="en">Developed by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2000.  TT2 ES cells were used.  The mutant mice were crossed to C57BL/6 over 6 times.</ns1:strainDevelopment>
    <ns0:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/mgi_rdf/MGI:4459527"/>
    <ns1:colonyMaintenance xml:lang="en">Hes3: Heterozygote x Wild-type [C57BL/6JJcl]; Hes5: Heterozygote x Wild-type [C57BL/6JJcl]</ns1:colonyMaintenance>
    <ns1:colonyMaintenance xml:lang="ja">Hes3: Heterozygote x Wild-type [C57BL/6JJcl]; Hes5: Heterozygote x Wild-type [C57BL/6JJcl]</ns1:colonyMaintenance>
    <ns5:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;研究成果の公表にあたって寄託者の指定する文献を引用する。Development. 2004 131(22):5539-50.&lt;br&gt;研究成果の公表にあたって謝辞の表明を必要とする。&lt;br&gt;学術機関の学術研究に限る。分与希望者は事前に 影山龍一郎 (E-mail: rkageyam@virus.kyoto-u.ac.jp) に連絡をとり、分与に関して許可を得ること。（提供承諾書（書式6）の提出は不要）。</ns5:termsAndConditions>
    <ns5:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;</ns5:addinfo>
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    <ns1:expectedDelivery xml:lang="ja">D（6か月以上）</ns1:expectedDelivery>
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